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1x mem non-essential amino acids solution (neaa)  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 1x mem non-essential amino acids solution (neaa)
    1x Mem Non Essential Amino Acids Solution (Neaa), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1x+mem+non-essential+amino+acids+solution/1%C3%97+mem+non+essential+amino+acids/pm40550888-195-22-35
    Average 90 stars, based on 1 article reviews
    1x mem non-essential amino acids solution (neaa) - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Cell Culture:

    Article Title: An Unbiased Approach to Identifying Cellular Reprogramming-Inducible Enhancers
    Article Snippet: HEK293T were cultured at 37 °C and 5% CO 2 concentration, in high-glucose DMEM (Sigma-Aldrich, Merck SA, Athens, Greece, D6429) supplemented with heat-inactivated FBS at 10% final concentration (Pan Biotech, Aidenbach, Germany, P30-1985), 1x GlutaMAXTM Supplement (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #35050-061), 1x MEM Non-Essential Amino Acids Solution (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #11140-050) and Penicillin–Streptomycin mix diluted at 1/100 (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #15140-122).

    Article Title: An Unbiased Approach to Identifying Cellular Reprogramming-Inducible Enhancers.
    Article Snippet: Upon the emergence of the first early iPSC colonies (around day 8), the medium was replaced with ESC medium: KnockOutTM DMEM (Gibco, Thermo Fisher Scientific, Waltham, MA, USA, #10829018), 20% Pansera FBS designed for ESCs (Pan Biotech, Aidenbach, Germany, P30-2602), 1x GlutaMAXTM Supplement (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #35050-061), 1x MEM Non-Essential Amino Acids Solution (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #11140-050), PenicillinStreptomycin mix diluted at 1/100 (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #15140-122) and 20 ng/mL mLIF (Santa Cruz Biotechnology, Dallas, Texas, USA, sc-4378).

    Article Title: The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma
    Article Snippet: All media were supplemented with 10% (v/v) fetal bovine serum (FBS) (Atlanta Biologicals, Cat# S11550), 100 units/ml penicillin, 100 μg/ml streptomycin (Gibco, Cat# 15140122), 1X MEM non-essential amino acids solution (Gibco, Cat# 11140050).

    Article Title: An Unbiased Approach to Identifying Cellular Reprogramming-Inducible Enhancers.
    Article Snippet: MEFs were cultured at 37 ◦C and 5% CO2 concentration, in high-glucose DMEM (Sigma-Aldrich, Merck SA, Athens, Greece, D6429) supplemented with heat-inactivated FBS at 10% final concentration (Pan Biotech, Aidenbach, Germany, P30-1985), 1x GlutaMAXTM Supplement (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #35050-061), 1x MEM Non-Essential Amino Acids Solution (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #11140-050) and Penicillin-Streptomycin mix diluted at 1/100 (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #15140-122).

    Article Title: Axial patterning of gastruloids via diverse cell type compositions
    Article Snippet: Base medium consists of KnockOut DMEM (ThermoFisher 10829018), 1x MEM Non-Essential Amino Acids Solution (100x, ThermoFisher 11140050), 1x GlutaMAX (100X, ThermoFisher 35050061), 1x Sodium Pyruvate (100x, ThermoFisher 11360070), 1x Pen/Strep (100x, ThermoFisher 15140122), 50μM B-mercaptoethanol (50mM, ThermoFisher 31350010), 10% fetal bovine serum (FBS) (batch tested) and home-made Leukemia Inhibitory Factor (LIF, also batch tested).

    Article Title: TDP-43 nuclear retention is antagonized by hypo-phosphorylation of its C-terminus in the cytoplasm.
    Article Snippet: Cell culture and plasmid co-transfection for MT bench experiments HeLa cells were obtained from ATCC and cultured at 37 °C, 5% CO2 in high-glucose DMEM medium (Dulbecco’s modified Eagle’s medium) supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine, 1x MEM non-essential amino acids solution and 1% penicillin–streptomycin (GIBCO Life Technologies).

    Article Title: Degradation rather than disassembly of necrotic debris is essential to enhance recovery after acute liver injury.
    Article Snippet: HepG2 cells were cultured at 37 °C and 5% CO2 atmosphere in high glucose Dulbecco’s Modified Eagle Medium (DMEM) with GlutaMAX (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), supplemented with 10% FBS (Sigma-Aldrich, Merck, Darmstadt, Germany), 20 mM HEPES buffer solution (Gibco) and 1X MEM Non-Essential Amino Acids Solution (Gibco).

    Article Title: Tailoring capsid-directed evolution technology for improved AAV-mediated CAR-T generation.
    Article Snippet: 1Translational Vectorology Research Unit, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney, Westmead, NSW, Australia; 2Infection, Immunity and Inflammation Research and Teaching Department, Great Ormond Street Institute of Child Health, University College, London, UK; 3Children’s Cancer Research Unit, Kids Research, The Children’s Hospital at Westmead, Faculty of Medicine and Health, The University of Sydney, Westmead, NSW, Australia; 4Australian e-Health Research Centre, Commonwealth Scientific and Industrial Research Organisation, Sydney, NSW, Australia; 5Vector and Genome Engineering Facility, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney, Westmead, NSW, Australia; 6Australian Genome Therapeutics Centre, Children’s Medical Research Institute and Sydney Children’s Hospitals Network, Westmead, NSW 2145, Australia; 7Laboratory of Molecular Oncology and Innovative Therapies, Military Institute of Medicine National Research Institute, Warsaw, Poland

    Modification:

    Article Title: An Unbiased Approach to Identifying Cellular Reprogramming-Inducible Enhancers
    Article Snippet: HEK293T were cultured at 37 °C and 5% CO 2 concentration, in high-glucose DMEM (Sigma-Aldrich, Merck SA, Athens, Greece, D6429) supplemented with heat-inactivated FBS at 10% final concentration (Pan Biotech, Aidenbach, Germany, P30-1985), 1x GlutaMAXTM Supplement (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #35050-061), 1x MEM Non-Essential Amino Acids Solution (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #11140-050) and Penicillin–Streptomycin mix diluted at 1/100 (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #15140-122).

    Article Title: An Unbiased Approach to Identifying Cellular Reprogramming-Inducible Enhancers.
    Article Snippet: Upon the emergence of the first early iPSC colonies (around day 8), the medium was replaced with ESC medium: KnockOutTM DMEM (Gibco, Thermo Fisher Scientific, Waltham, MA, USA, #10829018), 20% Pansera FBS designed for ESCs (Pan Biotech, Aidenbach, Germany, P30-2602), 1x GlutaMAXTM Supplement (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #35050-061), 1x MEM Non-Essential Amino Acids Solution (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #11140-050), PenicillinStreptomycin mix diluted at 1/100 (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #15140-122) and 20 ng/mL mLIF (Santa Cruz Biotechnology, Dallas, Texas, USA, sc-4378).

    Article Title: The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma
    Article Snippet: All media were supplemented with 10% (v/v) fetal bovine serum (FBS) (Atlanta Biologicals, Cat# S11550), 100 units/ml penicillin, 100 μg/ml streptomycin (Gibco, Cat# 15140122), 1X MEM non-essential amino acids solution (Gibco, Cat# 11140050).

    Article Title: An Unbiased Approach to Identifying Cellular Reprogramming-Inducible Enhancers.
    Article Snippet: MEFs were cultured at 37 ◦C and 5% CO2 concentration, in high-glucose DMEM (Sigma-Aldrich, Merck SA, Athens, Greece, D6429) supplemented with heat-inactivated FBS at 10% final concentration (Pan Biotech, Aidenbach, Germany, P30-1985), 1x GlutaMAXTM Supplement (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #35050-061), 1x MEM Non-Essential Amino Acids Solution (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #11140-050) and Penicillin-Streptomycin mix diluted at 1/100 (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #15140-122).

    Article Title: Axial patterning of gastruloids via diverse cell type compositions
    Article Snippet: Base medium consists of KnockOut DMEM (ThermoFisher 10829018), 1x MEM Non-Essential Amino Acids Solution (100x, ThermoFisher 11140050), 1x GlutaMAX (100X, ThermoFisher 35050061), 1x Sodium Pyruvate (100x, ThermoFisher 11360070), 1x Pen/Strep (100x, ThermoFisher 15140122), 50μM B-mercaptoethanol (50mM, ThermoFisher 31350010), 10% fetal bovine serum (FBS) (batch tested) and home-made Leukemia Inhibitory Factor (LIF, also batch tested).

    Article Title: TDP-43 nuclear retention is antagonized by hypo-phosphorylation of its C-terminus in the cytoplasm.
    Article Snippet: Cell culture and plasmid co-transfection for MT bench experiments HeLa cells were obtained from ATCC and cultured at 37 °C, 5% CO2 in high-glucose DMEM medium (Dulbecco’s modified Eagle’s medium) supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine, 1x MEM non-essential amino acids solution and 1% penicillin–streptomycin (GIBCO Life Technologies).

    Article Title: Degradation rather than disassembly of necrotic debris is essential to enhance recovery after acute liver injury.
    Article Snippet: HepG2 cells were cultured at 37 °C and 5% CO2 atmosphere in high glucose Dulbecco’s Modified Eagle Medium (DMEM) with GlutaMAX (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), supplemented with 10% FBS (Sigma-Aldrich, Merck, Darmstadt, Germany), 20 mM HEPES buffer solution (Gibco) and 1X MEM Non-Essential Amino Acids Solution (Gibco).

    Article Title: Tailoring capsid-directed evolution technology for improved AAV-mediated CAR-T generation.
    Article Snippet: 1Translational Vectorology Research Unit, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney, Westmead, NSW, Australia; 2Infection, Immunity and Inflammation Research and Teaching Department, Great Ormond Street Institute of Child Health, University College, London, UK; 3Children’s Cancer Research Unit, Kids Research, The Children’s Hospital at Westmead, Faculty of Medicine and Health, The University of Sydney, Westmead, NSW, Australia; 4Australian e-Health Research Centre, Commonwealth Scientific and Industrial Research Organisation, Sydney, NSW, Australia; 5Vector and Genome Engineering Facility, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney, Westmead, NSW, Australia; 6Australian Genome Therapeutics Centre, Children’s Medical Research Institute and Sydney Children’s Hospitals Network, Westmead, NSW 2145, Australia; 7Laboratory of Molecular Oncology and Innovative Therapies, Military Institute of Medicine National Research Institute, Warsaw, Poland

    Plasmid Preparation:

    Article Title: An Unbiased Approach to Identifying Cellular Reprogramming-Inducible Enhancers
    Article Snippet: HEK293T were cultured at 37 °C and 5% CO 2 concentration, in high-glucose DMEM (Sigma-Aldrich, Merck SA, Athens, Greece, D6429) supplemented with heat-inactivated FBS at 10% final concentration (Pan Biotech, Aidenbach, Germany, P30-1985), 1x GlutaMAXTM Supplement (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #35050-061), 1x MEM Non-Essential Amino Acids Solution (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #11140-050) and Penicillin–Streptomycin mix diluted at 1/100 (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #15140-122).

    Article Title: An Unbiased Approach to Identifying Cellular Reprogramming-Inducible Enhancers.
    Article Snippet: Upon the emergence of the first early iPSC colonies (around day 8), the medium was replaced with ESC medium: KnockOutTM DMEM (Gibco, Thermo Fisher Scientific, Waltham, MA, USA, #10829018), 20% Pansera FBS designed for ESCs (Pan Biotech, Aidenbach, Germany, P30-2602), 1x GlutaMAXTM Supplement (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #35050-061), 1x MEM Non-Essential Amino Acids Solution (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #11140-050), PenicillinStreptomycin mix diluted at 1/100 (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #15140-122) and 20 ng/mL mLIF (Santa Cruz Biotechnology, Dallas, Texas, USA, sc-4378).

    Article Title: The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma
    Article Snippet: All media were supplemented with 10% (v/v) fetal bovine serum (FBS) (Atlanta Biologicals, Cat# S11550), 100 units/ml penicillin, 100 μg/ml streptomycin (Gibco, Cat# 15140122), 1X MEM non-essential amino acids solution (Gibco, Cat# 11140050).

    Article Title: An Unbiased Approach to Identifying Cellular Reprogramming-Inducible Enhancers.
    Article Snippet: MEFs were cultured at 37 ◦C and 5% CO2 concentration, in high-glucose DMEM (Sigma-Aldrich, Merck SA, Athens, Greece, D6429) supplemented with heat-inactivated FBS at 10% final concentration (Pan Biotech, Aidenbach, Germany, P30-1985), 1x GlutaMAXTM Supplement (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #35050-061), 1x MEM Non-Essential Amino Acids Solution (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #11140-050) and Penicillin-Streptomycin mix diluted at 1/100 (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #15140-122).

    Article Title: Axial patterning of gastruloids via diverse cell type compositions
    Article Snippet: Base medium consists of KnockOut DMEM (ThermoFisher 10829018), 1x MEM Non-Essential Amino Acids Solution (100x, ThermoFisher 11140050), 1x GlutaMAX (100X, ThermoFisher 35050061), 1x Sodium Pyruvate (100x, ThermoFisher 11360070), 1x Pen/Strep (100x, ThermoFisher 15140122), 50μM B-mercaptoethanol (50mM, ThermoFisher 31350010), 10% fetal bovine serum (FBS) (batch tested) and home-made Leukemia Inhibitory Factor (LIF, also batch tested).

    Article Title: TDP-43 nuclear retention is antagonized by hypo-phosphorylation of its C-terminus in the cytoplasm.
    Article Snippet: Cell culture and plasmid co-transfection for MT bench experiments HeLa cells were obtained from ATCC and cultured at 37 °C, 5% CO2 in high-glucose DMEM medium (Dulbecco’s modified Eagle’s medium) supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine, 1x MEM non-essential amino acids solution and 1% penicillin–streptomycin (GIBCO Life Technologies).

    Article Title: Degradation rather than disassembly of necrotic debris is essential to enhance recovery after acute liver injury.
    Article Snippet: HepG2 cells were cultured at 37 °C and 5% CO2 atmosphere in high glucose Dulbecco’s Modified Eagle Medium (DMEM) with GlutaMAX (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), supplemented with 10% FBS (Sigma-Aldrich, Merck, Darmstadt, Germany), 20 mM HEPES buffer solution (Gibco) and 1X MEM Non-Essential Amino Acids Solution (Gibco).

    Article Title: Tailoring capsid-directed evolution technology for improved AAV-mediated CAR-T generation.
    Article Snippet: 1Translational Vectorology Research Unit, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney, Westmead, NSW, Australia; 2Infection, Immunity and Inflammation Research and Teaching Department, Great Ormond Street Institute of Child Health, University College, London, UK; 3Children’s Cancer Research Unit, Kids Research, The Children’s Hospital at Westmead, Faculty of Medicine and Health, The University of Sydney, Westmead, NSW, Australia; 4Australian e-Health Research Centre, Commonwealth Scientific and Industrial Research Organisation, Sydney, NSW, Australia; 5Vector and Genome Engineering Facility, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney, Westmead, NSW, Australia; 6Australian Genome Therapeutics Centre, Children’s Medical Research Institute and Sydney Children’s Hospitals Network, Westmead, NSW 2145, Australia; 7Laboratory of Molecular Oncology and Innovative Therapies, Military Institute of Medicine National Research Institute, Warsaw, Poland

    Knock-Out:

    Article Title: An Unbiased Approach to Identifying Cellular Reprogramming-Inducible Enhancers
    Article Snippet: HEK293T were cultured at 37 °C and 5% CO 2 concentration, in high-glucose DMEM (Sigma-Aldrich, Merck SA, Athens, Greece, D6429) supplemented with heat-inactivated FBS at 10% final concentration (Pan Biotech, Aidenbach, Germany, P30-1985), 1x GlutaMAXTM Supplement (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #35050-061), 1x MEM Non-Essential Amino Acids Solution (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #11140-050) and Penicillin–Streptomycin mix diluted at 1/100 (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #15140-122).

    Article Title: An Unbiased Approach to Identifying Cellular Reprogramming-Inducible Enhancers.
    Article Snippet: Upon the emergence of the first early iPSC colonies (around day 8), the medium was replaced with ESC medium: KnockOutTM DMEM (Gibco, Thermo Fisher Scientific, Waltham, MA, USA, #10829018), 20% Pansera FBS designed for ESCs (Pan Biotech, Aidenbach, Germany, P30-2602), 1x GlutaMAXTM Supplement (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #35050-061), 1x MEM Non-Essential Amino Acids Solution (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #11140-050), PenicillinStreptomycin mix diluted at 1/100 (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #15140-122) and 20 ng/mL mLIF (Santa Cruz Biotechnology, Dallas, Texas, USA, sc-4378).

    Article Title: The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma
    Article Snippet: All media were supplemented with 10% (v/v) fetal bovine serum (FBS) (Atlanta Biologicals, Cat# S11550), 100 units/ml penicillin, 100 μg/ml streptomycin (Gibco, Cat# 15140122), 1X MEM non-essential amino acids solution (Gibco, Cat# 11140050).

    Article Title: An Unbiased Approach to Identifying Cellular Reprogramming-Inducible Enhancers.
    Article Snippet: MEFs were cultured at 37 ◦C and 5% CO2 concentration, in high-glucose DMEM (Sigma-Aldrich, Merck SA, Athens, Greece, D6429) supplemented with heat-inactivated FBS at 10% final concentration (Pan Biotech, Aidenbach, Germany, P30-1985), 1x GlutaMAXTM Supplement (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #35050-061), 1x MEM Non-Essential Amino Acids Solution (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #11140-050) and Penicillin-Streptomycin mix diluted at 1/100 (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #15140-122).

    Article Title: Axial patterning of gastruloids via diverse cell type compositions
    Article Snippet: Base medium consists of KnockOut DMEM (ThermoFisher 10829018), 1x MEM Non-Essential Amino Acids Solution (100x, ThermoFisher 11140050), 1x GlutaMAX (100X, ThermoFisher 35050061), 1x Sodium Pyruvate (100x, ThermoFisher 11360070), 1x Pen/Strep (100x, ThermoFisher 15140122), 50μM B-mercaptoethanol (50mM, ThermoFisher 31350010), 10% fetal bovine serum (FBS) (batch tested) and home-made Leukemia Inhibitory Factor (LIF, also batch tested).

    Article Title: TDP-43 nuclear retention is antagonized by hypo-phosphorylation of its C-terminus in the cytoplasm.
    Article Snippet: Cell culture and plasmid co-transfection for MT bench experiments HeLa cells were obtained from ATCC and cultured at 37 °C, 5% CO2 in high-glucose DMEM medium (Dulbecco’s modified Eagle’s medium) supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine, 1x MEM non-essential amino acids solution and 1% penicillin–streptomycin (GIBCO Life Technologies).

    Article Title: Degradation rather than disassembly of necrotic debris is essential to enhance recovery after acute liver injury.
    Article Snippet: HepG2 cells were cultured at 37 °C and 5% CO2 atmosphere in high glucose Dulbecco’s Modified Eagle Medium (DMEM) with GlutaMAX (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), supplemented with 10% FBS (Sigma-Aldrich, Merck, Darmstadt, Germany), 20 mM HEPES buffer solution (Gibco) and 1X MEM Non-Essential Amino Acids Solution (Gibco).

    Article Title: Tailoring capsid-directed evolution technology for improved AAV-mediated CAR-T generation.
    Article Snippet: 1Translational Vectorology Research Unit, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney, Westmead, NSW, Australia; 2Infection, Immunity and Inflammation Research and Teaching Department, Great Ormond Street Institute of Child Health, University College, London, UK; 3Children’s Cancer Research Unit, Kids Research, The Children’s Hospital at Westmead, Faculty of Medicine and Health, The University of Sydney, Westmead, NSW, Australia; 4Australian e-Health Research Centre, Commonwealth Scientific and Industrial Research Organisation, Sydney, NSW, Australia; 5Vector and Genome Engineering Facility, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney, Westmead, NSW, Australia; 6Australian Genome Therapeutics Centre, Children’s Medical Research Institute and Sydney Children’s Hospitals Network, Westmead, NSW 2145, Australia; 7Laboratory of Molecular Oncology and Innovative Therapies, Military Institute of Medicine National Research Institute, Warsaw, Poland

    Concentration Assay:

    Article Title: An Unbiased Approach to Identifying Cellular Reprogramming-Inducible Enhancers
    Article Snippet: HEK293T were cultured at 37 °C and 5% CO 2 concentration, in high-glucose DMEM (Sigma-Aldrich, Merck SA, Athens, Greece, D6429) supplemented with heat-inactivated FBS at 10% final concentration (Pan Biotech, Aidenbach, Germany, P30-1985), 1x GlutaMAXTM Supplement (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #35050-061), 1x MEM Non-Essential Amino Acids Solution (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #11140-050) and Penicillin–Streptomycin mix diluted at 1/100 (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #15140-122).

    Article Title: An Unbiased Approach to Identifying Cellular Reprogramming-Inducible Enhancers.
    Article Snippet: Upon the emergence of the first early iPSC colonies (around day 8), the medium was replaced with ESC medium: KnockOutTM DMEM (Gibco, Thermo Fisher Scientific, Waltham, MA, USA, #10829018), 20% Pansera FBS designed for ESCs (Pan Biotech, Aidenbach, Germany, P30-2602), 1x GlutaMAXTM Supplement (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #35050-061), 1x MEM Non-Essential Amino Acids Solution (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #11140-050), PenicillinStreptomycin mix diluted at 1/100 (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #15140-122) and 20 ng/mL mLIF (Santa Cruz Biotechnology, Dallas, Texas, USA, sc-4378).

    Article Title: The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma
    Article Snippet: All media were supplemented with 10% (v/v) fetal bovine serum (FBS) (Atlanta Biologicals, Cat# S11550), 100 units/ml penicillin, 100 μg/ml streptomycin (Gibco, Cat# 15140122), 1X MEM non-essential amino acids solution (Gibco, Cat# 11140050).

    Article Title: An Unbiased Approach to Identifying Cellular Reprogramming-Inducible Enhancers.
    Article Snippet: MEFs were cultured at 37 ◦C and 5% CO2 concentration, in high-glucose DMEM (Sigma-Aldrich, Merck SA, Athens, Greece, D6429) supplemented with heat-inactivated FBS at 10% final concentration (Pan Biotech, Aidenbach, Germany, P30-1985), 1x GlutaMAXTM Supplement (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #35050-061), 1x MEM Non-Essential Amino Acids Solution (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #11140-050) and Penicillin-Streptomycin mix diluted at 1/100 (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #15140-122).

    Article Title: Axial patterning of gastruloids via diverse cell type compositions
    Article Snippet: Base medium consists of KnockOut DMEM (ThermoFisher 10829018), 1x MEM Non-Essential Amino Acids Solution (100x, ThermoFisher 11140050), 1x GlutaMAX (100X, ThermoFisher 35050061), 1x Sodium Pyruvate (100x, ThermoFisher 11360070), 1x Pen/Strep (100x, ThermoFisher 15140122), 50μM B-mercaptoethanol (50mM, ThermoFisher 31350010), 10% fetal bovine serum (FBS) (batch tested) and home-made Leukemia Inhibitory Factor (LIF, also batch tested).

    Article Title: TDP-43 nuclear retention is antagonized by hypo-phosphorylation of its C-terminus in the cytoplasm.
    Article Snippet: Cell culture and plasmid co-transfection for MT bench experiments HeLa cells were obtained from ATCC and cultured at 37 °C, 5% CO2 in high-glucose DMEM medium (Dulbecco’s modified Eagle’s medium) supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine, 1x MEM non-essential amino acids solution and 1% penicillin–streptomycin (GIBCO Life Technologies).

    Article Title: Degradation rather than disassembly of necrotic debris is essential to enhance recovery after acute liver injury.
    Article Snippet: HepG2 cells were cultured at 37 °C and 5% CO2 atmosphere in high glucose Dulbecco’s Modified Eagle Medium (DMEM) with GlutaMAX (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), supplemented with 10% FBS (Sigma-Aldrich, Merck, Darmstadt, Germany), 20 mM HEPES buffer solution (Gibco) and 1X MEM Non-Essential Amino Acids Solution (Gibco).

    Article Title: Tailoring capsid-directed evolution technology for improved AAV-mediated CAR-T generation.
    Article Snippet: 1Translational Vectorology Research Unit, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney, Westmead, NSW, Australia; 2Infection, Immunity and Inflammation Research and Teaching Department, Great Ormond Street Institute of Child Health, University College, London, UK; 3Children’s Cancer Research Unit, Kids Research, The Children’s Hospital at Westmead, Faculty of Medicine and Health, The University of Sydney, Westmead, NSW, Australia; 4Australian e-Health Research Centre, Commonwealth Scientific and Industrial Research Organisation, Sydney, NSW, Australia; 5Vector and Genome Engineering Facility, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney, Westmead, NSW, Australia; 6Australian Genome Therapeutics Centre, Children’s Medical Research Institute and Sydney Children’s Hospitals Network, Westmead, NSW 2145, Australia; 7Laboratory of Molecular Oncology and Innovative Therapies, Military Institute of Medicine National Research Institute, Warsaw, Poland

    Cytotoxicity Assay:

    Article Title: An Unbiased Approach to Identifying Cellular Reprogramming-Inducible Enhancers
    Article Snippet: HEK293T were cultured at 37 °C and 5% CO 2 concentration, in high-glucose DMEM (Sigma-Aldrich, Merck SA, Athens, Greece, D6429) supplemented with heat-inactivated FBS at 10% final concentration (Pan Biotech, Aidenbach, Germany, P30-1985), 1x GlutaMAXTM Supplement (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #35050-061), 1x MEM Non-Essential Amino Acids Solution (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #11140-050) and Penicillin–Streptomycin mix diluted at 1/100 (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #15140-122).

    Article Title: An Unbiased Approach to Identifying Cellular Reprogramming-Inducible Enhancers.
    Article Snippet: Upon the emergence of the first early iPSC colonies (around day 8), the medium was replaced with ESC medium: KnockOutTM DMEM (Gibco, Thermo Fisher Scientific, Waltham, MA, USA, #10829018), 20% Pansera FBS designed for ESCs (Pan Biotech, Aidenbach, Germany, P30-2602), 1x GlutaMAXTM Supplement (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #35050-061), 1x MEM Non-Essential Amino Acids Solution (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #11140-050), PenicillinStreptomycin mix diluted at 1/100 (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #15140-122) and 20 ng/mL mLIF (Santa Cruz Biotechnology, Dallas, Texas, USA, sc-4378).

    Article Title: The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma
    Article Snippet: All media were supplemented with 10% (v/v) fetal bovine serum (FBS) (Atlanta Biologicals, Cat# S11550), 100 units/ml penicillin, 100 μg/ml streptomycin (Gibco, Cat# 15140122), 1X MEM non-essential amino acids solution (Gibco, Cat# 11140050).

    Article Title: An Unbiased Approach to Identifying Cellular Reprogramming-Inducible Enhancers.
    Article Snippet: MEFs were cultured at 37 ◦C and 5% CO2 concentration, in high-glucose DMEM (Sigma-Aldrich, Merck SA, Athens, Greece, D6429) supplemented with heat-inactivated FBS at 10% final concentration (Pan Biotech, Aidenbach, Germany, P30-1985), 1x GlutaMAXTM Supplement (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #35050-061), 1x MEM Non-Essential Amino Acids Solution (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #11140-050) and Penicillin-Streptomycin mix diluted at 1/100 (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #15140-122).

    Article Title: Axial patterning of gastruloids via diverse cell type compositions
    Article Snippet: Base medium consists of KnockOut DMEM (ThermoFisher 10829018), 1x MEM Non-Essential Amino Acids Solution (100x, ThermoFisher 11140050), 1x GlutaMAX (100X, ThermoFisher 35050061), 1x Sodium Pyruvate (100x, ThermoFisher 11360070), 1x Pen/Strep (100x, ThermoFisher 15140122), 50μM B-mercaptoethanol (50mM, ThermoFisher 31350010), 10% fetal bovine serum (FBS) (batch tested) and home-made Leukemia Inhibitory Factor (LIF, also batch tested).

    Article Title: TDP-43 nuclear retention is antagonized by hypo-phosphorylation of its C-terminus in the cytoplasm.
    Article Snippet: Cell culture and plasmid co-transfection for MT bench experiments HeLa cells were obtained from ATCC and cultured at 37 °C, 5% CO2 in high-glucose DMEM medium (Dulbecco’s modified Eagle’s medium) supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine, 1x MEM non-essential amino acids solution and 1% penicillin–streptomycin (GIBCO Life Technologies).

    Article Title: Degradation rather than disassembly of necrotic debris is essential to enhance recovery after acute liver injury.
    Article Snippet: HepG2 cells were cultured at 37 °C and 5% CO2 atmosphere in high glucose Dulbecco’s Modified Eagle Medium (DMEM) with GlutaMAX (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), supplemented with 10% FBS (Sigma-Aldrich, Merck, Darmstadt, Germany), 20 mM HEPES buffer solution (Gibco) and 1X MEM Non-Essential Amino Acids Solution (Gibco).

    Article Title: Tailoring capsid-directed evolution technology for improved AAV-mediated CAR-T generation.
    Article Snippet: 1Translational Vectorology Research Unit, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney, Westmead, NSW, Australia; 2Infection, Immunity and Inflammation Research and Teaching Department, Great Ormond Street Institute of Child Health, University College, London, UK; 3Children’s Cancer Research Unit, Kids Research, The Children’s Hospital at Westmead, Faculty of Medicine and Health, The University of Sydney, Westmead, NSW, Australia; 4Australian e-Health Research Centre, Commonwealth Scientific and Industrial Research Organisation, Sydney, NSW, Australia; 5Vector and Genome Engineering Facility, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney, Westmead, NSW, Australia; 6Australian Genome Therapeutics Centre, Children’s Medical Research Institute and Sydney Children’s Hospitals Network, Westmead, NSW 2145, Australia; 7Laboratory of Molecular Oncology and Innovative Therapies, Military Institute of Medicine National Research Institute, Warsaw, Poland

    Recombinant:

    Article Title: An Unbiased Approach to Identifying Cellular Reprogramming-Inducible Enhancers
    Article Snippet: HEK293T were cultured at 37 °C and 5% CO 2 concentration, in high-glucose DMEM (Sigma-Aldrich, Merck SA, Athens, Greece, D6429) supplemented with heat-inactivated FBS at 10% final concentration (Pan Biotech, Aidenbach, Germany, P30-1985), 1x GlutaMAXTM Supplement (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #35050-061), 1x MEM Non-Essential Amino Acids Solution (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #11140-050) and Penicillin–Streptomycin mix diluted at 1/100 (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #15140-122).

    Article Title: An Unbiased Approach to Identifying Cellular Reprogramming-Inducible Enhancers.
    Article Snippet: Upon the emergence of the first early iPSC colonies (around day 8), the medium was replaced with ESC medium: KnockOutTM DMEM (Gibco, Thermo Fisher Scientific, Waltham, MA, USA, #10829018), 20% Pansera FBS designed for ESCs (Pan Biotech, Aidenbach, Germany, P30-2602), 1x GlutaMAXTM Supplement (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #35050-061), 1x MEM Non-Essential Amino Acids Solution (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #11140-050), PenicillinStreptomycin mix diluted at 1/100 (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #15140-122) and 20 ng/mL mLIF (Santa Cruz Biotechnology, Dallas, Texas, USA, sc-4378).

    Article Title: The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma
    Article Snippet: All media were supplemented with 10% (v/v) fetal bovine serum (FBS) (Atlanta Biologicals, Cat# S11550), 100 units/ml penicillin, 100 μg/ml streptomycin (Gibco, Cat# 15140122), 1X MEM non-essential amino acids solution (Gibco, Cat# 11140050).

    Article Title: An Unbiased Approach to Identifying Cellular Reprogramming-Inducible Enhancers.
    Article Snippet: MEFs were cultured at 37 ◦C and 5% CO2 concentration, in high-glucose DMEM (Sigma-Aldrich, Merck SA, Athens, Greece, D6429) supplemented with heat-inactivated FBS at 10% final concentration (Pan Biotech, Aidenbach, Germany, P30-1985), 1x GlutaMAXTM Supplement (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #35050-061), 1x MEM Non-Essential Amino Acids Solution (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #11140-050) and Penicillin-Streptomycin mix diluted at 1/100 (GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA, #15140-122).

    Article Title: Axial patterning of gastruloids via diverse cell type compositions
    Article Snippet: Base medium consists of KnockOut DMEM (ThermoFisher 10829018), 1x MEM Non-Essential Amino Acids Solution (100x, ThermoFisher 11140050), 1x GlutaMAX (100X, ThermoFisher 35050061), 1x Sodium Pyruvate (100x, ThermoFisher 11360070), 1x Pen/Strep (100x, ThermoFisher 15140122), 50μM B-mercaptoethanol (50mM, ThermoFisher 31350010), 10% fetal bovine serum (FBS) (batch tested) and home-made Leukemia Inhibitory Factor (LIF, also batch tested).

    Article Title: TDP-43 nuclear retention is antagonized by hypo-phosphorylation of its C-terminus in the cytoplasm.
    Article Snippet: Cell culture and plasmid co-transfection for MT bench experiments HeLa cells were obtained from ATCC and cultured at 37 °C, 5% CO2 in high-glucose DMEM medium (Dulbecco’s modified Eagle’s medium) supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine, 1x MEM non-essential amino acids solution and 1% penicillin–streptomycin (GIBCO Life Technologies).

    Article Title: Degradation rather than disassembly of necrotic debris is essential to enhance recovery after acute liver injury.
    Article Snippet: HepG2 cells were cultured at 37 °C and 5% CO2 atmosphere in high glucose Dulbecco’s Modified Eagle Medium (DMEM) with GlutaMAX (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), supplemented with 10% FBS (Sigma-Aldrich, Merck, Darmstadt, Germany), 20 mM HEPES buffer solution (Gibco) and 1X MEM Non-Essential Amino Acids Solution (Gibco).

    Article Title: Tailoring capsid-directed evolution technology for improved AAV-mediated CAR-T generation.
    Article Snippet: 1Translational Vectorology Research Unit, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney, Westmead, NSW, Australia; 2Infection, Immunity and Inflammation Research and Teaching Department, Great Ormond Street Institute of Child Health, University College, London, UK; 3Children’s Cancer Research Unit, Kids Research, The Children’s Hospital at Westmead, Faculty of Medicine and Health, The University of Sydney, Westmead, NSW, Australia; 4Australian e-Health Research Centre, Commonwealth Scientific and Industrial Research Organisation, Sydney, NSW, Australia; 5Vector and Genome Engineering Facility, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney, Westmead, NSW, Australia; 6Australian Genome Therapeutics Centre, Children’s Medical Research Institute and Sydney Children’s Hospitals Network, Westmead, NSW 2145, Australia; 7Laboratory of Molecular Oncology and Innovative Therapies, Military Institute of Medicine National Research Institute, Warsaw, Poland



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